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Skeletal Muscle Neurovascular Coupling, Oxidative Capacity, and Microvascular Function with 'One Stop Shop' Near-infrared Spectroscopy
Published on: February 20, 2018
Active muscle oxygenation dynamics measured during high-intensity exercise by using two near-infrared spectroscopy
Tadashi Saitoh1, Anna Ooue, Narihiko Kondo
1Department of Bio-System Engineering,Graduate School of Science and Engineering, Yamagata University, 992-0038 Yonezawa, Japan. saitoh-t@yz.yamagata-u.ac.jp
Advances in Experimental Medicine and Biology
|March 6, 2010
Summary
Time-resolved spectroscopy (TRS) measures absolute muscle oxygenation changes during exercise, unlike modified Beer-Lambert
Area of Science:
- Physiology
- Biomedical Optics
- Exercise Science
Background:
- Near-infrared spectroscopy (NIRS) monitors tissue oxygenation noninvasively.
- Modified Beer-Lambert (MBL) law with continuous-wave light measures muscle oxygenation during exercise but not absolute concentrations.
- Time-resolved spectroscopy (TRS) with pulsed lasers measures absolute concentrations but has lower temporal resolution than MBL.
Purpose of the Study:
- To evaluate absolute changes in muscle oxygenation during high-intensity exercise using TRS.
- To determine optical properties like mean pathlength and scattering/absorption coefficients during exercise.
- To compare TRS-derived changes with MBL measurements.
Main Methods:
- Utilized a time-resolved spectroscopy (TRS) system.
- Measured absolute changes in oxy-, deoxy-, and total hemoglobin/myoglobin (Hb+Mb) concentrations.
- Assessed optical mean pathlength, scattering, and absorption coefficients.
Main Results:
- TRS provided absolute concentration changes, unlike MBL.
- Significant differences were observed in total-[Hb+Mb] and oxy-[Hb+Mb] dynamics between TRS and MBL during high-intensity exercise.
- Deoxy-[Hb+Mb] dynamics and kinetics showed no significant difference between the two methods.
Conclusions:
- TRS enables accurate assessment of absolute muscle oxygenation and optical properties during exercise.
- MBL may underestimate or misrepresent dynamic changes in oxygenation compared to TRS.
- TRS is crucial for precise physiological monitoring of muscle oxygenation during intense physical activity.
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