Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Flow Cytometry

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
In...

Confocal Fluorescence Microscopy

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Rate and Predictors of Urine Culture Positivity Following Single-Dose Antibiotic Administration: a Prospective Single-Center Study.

Infectious diseases and therapy·2025

Same author

Spectrally encoded flow cytometry using few-mode fiber collection.

Biomedical optics express·2025

Same author

Measuring the Acoustic Reflex through the Tympanic Membrane.

Audiology & neuro-otology·2024

Same author

Pinhole shifting for reducing speckle contrast in reflectance confocal microscopy.

Optics letters·2023

Same author

In vivo optical mapping of the tympanic membrane impulse response.

Hearing research·2023

Same author

Measuring the red blood cell shape in capillary flow using spectrally encoded flow cytometry.

Biomedical optics express·2022

Same journal

Gaussian-modulated continuous-variable quantum key distribution over 60 km fiber using an integrated silicon photonic receiver.

Optics letters·2026

Same journal

E2E-OCT: end-to-end joint learning model using optical coherence tomography images for vocal cord leukoplakia diagnosis.

Optics letters·2026

Same journal

Holographic generation of panoramic 3D scenes by concave ellipsoidal mirror reflection.

Optics letters·2026

Same journal

Dual-pilot phase recovery with pair-wise maximum-ratio combining for coherent PONs.

Optics letters·2026

Same journal

Mapping the whispering gallery modes of a CaF<sub>2</sub> disk resonator with half-tapered fibers to estimate the fundamental mode volume.

Optics letters·2026

Same journal

Quantitative estimation of deep-subwavelength scale via dark-field scattering axial energy concentration decay profiles.

Optics letters·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Jun 11, 2026

Analysis of Cell Suspensions Isolated from Solid Tissues by Spectral Flow Cytometry

Analysis of Cell Suspensions Isolated from Solid Tissues by Spectral Flow Cytometry

Published on: May 5, 2017

Flow cytometry using spectrally encoded confocal microscopy.

Lior Golan¹, Dvir Yelin

¹Faculty of Biomedical Engineering, Technion-Israel Institute of Technology, Haifa 32000, Israel. liorgo@tx.technion.ac.il

|July 3, 2010

Summary

This summary is machine-generated.

This study introduces a novel confocal imaging technique for unstained cells, overcoming limitations of traditional flow cytometry in resolving cell clusters and parallel flow. The method enables detailed imaging of cells in wide channels without mechanical scanning.

More Related Videos

High-plex Imaging using Spectral Confocal Microscopy to Minimize Non-specific Tissue Fluorescence

High-plex Imaging using Spectral Confocal Microscopy to Minimize Non-specific Tissue Fluorescence

Published on: October 28, 2025

Confocal Microscopy Reveals Cell Surface Receptor Aggregation Through Image Correlation Spectroscopy

Confocal Microscopy Reveals Cell Surface Receptor Aggregation Through Image Correlation Spectroscopy

Published on: August 2, 2018

Related Experiment Videos

Last Updated: Jun 11, 2026

Analysis of Cell Suspensions Isolated from Solid Tissues by Spectral Flow Cytometry

Analysis of Cell Suspensions Isolated from Solid Tissues by Spectral Flow Cytometry

Published on: May 5, 2017

High-plex Imaging using Spectral Confocal Microscopy to Minimize Non-specific Tissue Fluorescence

High-plex Imaging using Spectral Confocal Microscopy to Minimize Non-specific Tissue Fluorescence

Published on: October 28, 2025

Confocal Microscopy Reveals Cell Surface Receptor Aggregation Through Image Correlation Spectroscopy

Confocal Microscopy Reveals Cell Surface Receptor Aggregation Through Image Correlation Spectroscopy

Published on: August 2, 2018

Area of Science:

Biomedical Engineering
Optical Microscopy
Cellular Imaging

Background:

Flow cytometry is crucial for analyzing large cell populations but struggles with resolving cell clusters and parallel flow in wider vessels.
Existing methods often require cell staining or mechanical scanning, limiting throughput and applicability.

Purpose of the Study:

To develop and demonstrate a novel confocal imaging technique for unstained cells in parallel flow.
To overcome the limitations of conventional flow cytometry in resolving cellular structures and flow dynamics.

Main Methods:

Development of spectrally encoded reflectance confocal microscopy without mechanical scanning.
Imaging of unstained cells, specifically red blood cells, flowing in a wide channel.
Comparison of generated images with conventional transmission microscopy.

Main Results:

Successful imaging of unstained red blood cells flowing in parallel within a wide channel.
Demonstration of the technique's ability to visualize cellular details without staining.
Experimental validation of the in vitro imaging of flowing red blood cells.

Conclusions:

The developed confocal imaging technique offers a viable alternative to flow cytometry for specific applications.
This method enhances the ability to study cell clusters and parallel flow dynamics in unstained samples.
The system provides high-resolution imaging of flowing cells without the need for mechanical scanning or staining.