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Related Concept Videos

Types Of Column Chromatography01:29

Types Of Column Chromatography

The stability and compatibility of column material with samples are crucial for efficient purification in chromatographic techniques. Various operating parameters such as pH, temperature, or solvent affect the packing of the column material, thereby determining the purification efficiency. The choice of column material also plays an essential role in deciding the operating parameters and can be modified based on the proteins that need to be purified.
Gel Filtration Chromatography
When the...
Principles Of Column Chromatography01:13

Principles Of Column Chromatography

The chromatography technique was first invented in 1901 by Michael S. Tswett, a Russian botanist, to separate plant pigments using organic solvents. Further, in 1941, Archer John Porter Martin and R. L. M. Synge modified the technique by packing silica gel into a column. A mixture of amino acids was then separated on the packed column using chloroform and water mixture as the mobile phase. This was the first report on column chromatography. At present, column chromatography is a widely used...
Size-Exclusion Chromatography01:08

Size-Exclusion Chromatography

In size-exclusion chromatography (SEC), also known as molecular-exclusion or gel-permeation chromatography, molecules are separated based on their sizes. This technique is important for separating large molecules such as polymers and biomolecules. The two classes of micron-sized stationary phases encountered in SEC are silica particles and cross-linked polymer resin beads. Both materials are porous, but their pore sizes vary significantly.
Silica particles offer advantages such as rigidity,...
High-Performance Liquid Chromatography: Elution Process01:05

High-Performance Liquid Chromatography: Elution Process

In High-Performance Liquid Chromatography (HPLC), the elution process is critical to the separation of analytes and the quality of chromatographic results. Elution describes how compounds move through the column and separate based on their interactions with the mobile and stationary phases. This process determines the resolution, peak shape, and retention times in the chromatogram, which are essential for identifying and quantifying components in complex mixtures. Understanding the elution...
Capillary Electrophoresis: Applications01:30

Capillary Electrophoresis: Applications

Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...

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Updated: Jun 7, 2026

Digital Microfluidics for Automated Proteomic Processing
10:55

Digital Microfluidics for Automated Proteomic Processing

Published on: November 6, 2009

Microfluidics in protein chromatography.

Frank A Gomez1

  • 1Department of Chemistry and Biochemistry, California State University, Los Angeles, CA, USA.

Methods in Molecular Biology (Clifton, N.J.)
|October 28, 2010
PubMed
Summary
This summary is machine-generated.

Microfluidic platforms and microelectromechanical systems (MEMS) are advancing protein separations. This review explores recent developments in microchip-based protein chromatography, highlighting their potential to revolutionize the field.

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Last Updated: Jun 7, 2026

Digital Microfluidics for Automated Proteomic Processing
10:55

Digital Microfluidics for Automated Proteomic Processing

Published on: November 6, 2009

Fast Enzymatic Processing of Proteins for MS Detection with a Flow-through Microreactor
09:49

Fast Enzymatic Processing of Proteins for MS Detection with a Flow-through Microreactor

Published on: April 6, 2016

A Microfluidic Chip for ICPMS Sample Introduction
11:16

A Microfluidic Chip for ICPMS Sample Introduction

Published on: March 5, 2015

Area of Science:

  • Biochemistry
  • Analytical Chemistry
  • Microfluidics

Background:

  • Microfluidics technology has advanced significantly over the last 15 years.
  • Microelectromechanical systems (MEMS) fabrication has enabled progress in separations chemistry.
  • Existing research primarily focuses on small molecule and DNA separations, with limited exploration of protein chromatography on microchips.

Purpose of the Study:

  • To review recent advancements in protein separations utilizing microfluidic platforms.
  • To explore the potential of MEMS in revolutionizing protein chromatography.

Main Methods:

  • Review of recent literature on microfluidic protein separation techniques.
  • Analysis of microelectromechanical systems (MEMS) applications in microchip chromatography for proteins.

Main Results:

  • Microfluidic platforms show promise for efficient protein separations.
  • MEMS technology offers novel approaches for miniaturized protein chromatography.

Conclusions:

  • Microfluidic-based protein chromatography is an emerging field with significant potential.
  • MEMS fabrication techniques are key to developing next-generation protein separation technologies on microchips.