Related Experiment Video
Updated: Jun 5, 2026
Primer for Immunohistochemistry on Cryosectioned Rat Brain Tissue: Example Staining for Microglia and Neurons
Published on: May 12, 2015
The use of immunohistochemical method for detection of brain microglia in paraffin sections
E G Suchorukova1, O V Kirik, D E Korzhevskii
1Department of General and Special Morphology, Institute of Experimental Medicine, Russian Academy of Medical Sciences, St. Petersburg, Russia.
Abstract:
We developed a well reproducible method for detection of brain microglia on paraffin sections on the basis of modern immunohistochemical methods. Polyclonal antibodies to Iba1 antigen obtained after immunization with a synthetic peptide corresponding to 81-93 amino acid fragment of Iba1 antigen selectively interact with microglia cells on paraffin sections of rat brain. Detailed protocol of preparation treatment was drawn up ensuring complete detection of the microglia expressing Iba1 protein.
Insights
We developed a reproducible method to detect brain microglia using immunohistochemistry on paraffin sections. This technique reliably identifies microglia expressing the Iba1 protein in rat brain tissue.
Area of Science:
- Neuroscience
- Immunohistochemistry
Background:
- Microglia are key immune cells in the brain.
- Accurate detection of microglia is essential for neurological research.
Purpose of the Study:
- To establish a reproducible immunohistochemical method for detecting microglia in paraffin-embedded rat brain sections.
- To optimize the detection of microglia expressing the Iba1 protein.
Main Methods:
- Utilized modern immunohistochemical techniques.
- Developed a protocol involving polyclonal antibodies against the Iba1 antigen (amino acids 81-93).
- Applied the method to paraffin-embedded rat brain sections.
Main Results:
- Achieved highly reproducible detection of microglia.
- Demonstrated selective interaction of antibodies with microglia expressing Iba1.
- Ensured complete detection of Iba1-expressing microglia.
Conclusions:
- The developed method provides reliable microglia detection in paraffin sections.
- This technique is valuable for studying microglia in neuropathology.
- The protocol ensures comprehensive identification of Iba1-positive microglia.
More Related Videos
10:40Immunofluorescence Staining Using IBA1 and TMEM119 for Microglial Density, Morphology and Peripheral Myeloid Cell Infiltration Analysis in Mouse Brain
Published on: October 27, 2019
08:44Quantifying Microglia Morphology from Photomicrographs of Immunohistochemistry Prepared Tissue Using ImageJ
Published on: June 5, 2018