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Related Concept Videos

Real Time RT-PCR02:57

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...

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Related Experiment Video

Updated: May 26, 2026

Probe-based Real-time PCR Approaches for Quantitative Measurement of microRNAs
10:28

Probe-based Real-time PCR Approaches for Quantitative Measurement of microRNAs

Published on: April 14, 2015

Stem-loop RT-qPCR for microRNA expression profiling.

James Hurley1, Doug Roberts, Andrew Bond

  • 1Life Technologies Corporation, Foster City, CA, USA.

Methods in Molecular Biology (Clifton, N.J.)
|December 7, 2011
PubMed
Summary
This summary is machine-generated.

Accurately quantifying microRNAs (miRNAs) is vital for biological research. This study showcases TaqMan Array Card and OpenArray platforms for precise miRNA expression analysis in biological samples.

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Profiling of Pre-micro RNAs and microRNAs using Quantitative Real-time PCR (qPCR) Arrays
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Profiling of Pre-micro RNAs and microRNAs using Quantitative Real-time PCR (qPCR) Arrays

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Related Experiment Videos

Last Updated: May 26, 2026

Probe-based Real-time PCR Approaches for Quantitative Measurement of microRNAs
10:28

Probe-based Real-time PCR Approaches for Quantitative Measurement of microRNAs

Published on: April 14, 2015

High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs
07:27

High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs

Published on: August 3, 2011

Profiling of Pre-micro RNAs and microRNAs using Quantitative Real-time PCR (qPCR) Arrays
10:58

Profiling of Pre-micro RNAs and microRNAs using Quantitative Real-time PCR (qPCR) Arrays

Published on: December 3, 2010

Area of Science:

  • Molecular Biology
  • Genetics
  • Biochemistry

Background:

  • Accurate quantification of microRNAs (miRNAs) is essential for understanding their biological roles.
  • Quantitative PCR (qPCR) combined with stem-loop reverse transcription and TaqMan assays provides reliable miRNA detection.
  • High-throughput methods are needed for screening numerous miRNAs from limited biological samples.

Purpose of the Study:

  • To demonstrate the utility of TaqMan Array Card and OpenArray platforms for miRNA quantification.
  • To validate these platforms for accurate measurement of miRNA gene expression levels.
  • To highlight the efficiency of these platforms for high-throughput miRNA screening.

Main Methods:

  • Utilized stem-loop reverse transcription and TaqMan miRNA assays.
  • Employed TaqMan Array Card platform for miRNA quantification.
  • Applied OpenArray platform for miRNA gene expression analysis in biological samples.

Main Results:

  • Both TaqMan Array Card and OpenArray platforms accurately determined miRNA expression levels.
  • The platforms enabled high-throughput screening of multiple miRNAs.
  • Reliable quantification was achieved even with small amounts of biological sample.

Conclusions:

  • TaqMan Array Card and OpenArray platforms are effective tools for accurate miRNA quantification.
  • These platforms facilitate efficient and high-throughput miRNA expression profiling.
  • The study validates advanced methods for miRNA analysis in biological research.