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Gene Trapping Using Gal4 in Zebrafish
Published on: September 29, 2013
Zebrafish Enhancer TRAP transgenic line database ZETRAP 2.0.
Igor Kondrychyn1, Cathleen Teh, Marta Garcia-Lecea
1Institute of Molecular and Cell Biology, Singapore.
Zebrafish
|December 21, 2011
Summary
The Zebrafish Enhancer TRAP lines database (ZETRAP) now includes new transgenic zebrafish lines expressing KillerRed protein for enhanced bioimaging and optogenetics. ZETRAP 2.0 offers expanded genomic data and expression patterns for these valuable research tools.
Area of Science:
- Developmental Biology
- Genomics
- Bioimaging
Background:
- The initial Zebrafish Enhancer TRAP lines database (ZETRAP) provided data on transgenic zebrafish expressing cytosolic EGFP.
- Transgenic lines were generated using Tol2 transposon-mediated transgenesis and subsequent transposon "rejump" strategies.
Purpose of the Study:
- To update and expand the ZETRAP database with new transgenic lines and comprehensive genomic information.
- To introduce transgenic lines expressing the membrane-tethered KillerRed (KR) protein for advanced applications.
Main Methods:
- Generation of transgenic zebrafish lines using Tol2 transposon-mediated transgenesis.
- Systematic "rejump" of transposons to create multiple transgenic lines.
- Expression of cytosolic EGFP and membrane-tethered KillerRed (KR) proteins.
- Compilation of genomic data, flanking insertion site sequences, and expression patterns.
Main Results:
- The updated Zebrafish Enhancer TRAP lines database version 2.0 (ZETRAP 2.0) has been created.
- New transgenic lines expressing KillerRed (KR) protein have been generated.
- ZETRAP 2.0 includes genomic insertion site data, gene links, and confocal images.
Conclusions:
- ZETRAP 2.0 is an expanded resource for zebrafish transgenic lines.
- KillerRed (KR) transgenic lines offer complementary utility to GFP lines for bioimaging.
- KR transgenics have potential applications in tissue-specific optogenetics.
