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Flow Cytometry

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Updated: May 26, 2026

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells

Published on: October 15, 2013

Micro segmented-flow in biochemical and cell-based assays.

Jenifer Clausell-Tormos¹, Christoph A Merten

¹Centro Nacional de Investigaciones Oncologicas, C/ Melchor Fernandez Almagro 3, Madrid, 28029 Spain.

Frontiers in Bioscience (Elite Edition)

|December 29, 2011

Summary

This summary is machine-generated.

Micro-segmented flow assays offer significant cost reduction and high resolution for biological research. This technique enables massive sample volume reduction, ideal for low-quantity samples and high-throughput biological analyses.

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Area of Science:

Biotechnology
Analytical Chemistry
Cell Biology

Background:

Micro-segmented flow is an emerging technique in modern biology.
It offers advantages over conventional microtiter plate formats.

Purpose of the Study:

To review the state-of-the-art of micro-segmented flow in biological assays.
To discuss future applications and compare with droplet-based methods.

Main Methods:

Review of current literature on micro-segmented flow applications.
Analysis of biochemical, cell-based, and multicellular organism assays.
Comparison with emulsion-based (droplet-based) approaches.

Main Results:

Micro-segmented flow enables ~1000-fold reduction in sample volumes.
Assays benefit from reduced costs and suitability for low-quantity samples (e.g., primary cells).
High parallelization and superb spatio-temporal resolution are achievable.

Conclusions:

Micro-segmented flow is a powerful technique for various biological assays.
Future applications include single-cell/organism proteomics and transcriptomics.
It presents specific advantages and limitations compared to droplet-based methods.