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Related Concept Videos

Immunocytochemistry and Immunohistochemistry01:22

Immunocytochemistry and Immunohistochemistry

Immunocytochemistry (ICC) and immunohistochemistry (IHC) are techniques that use antibodies to check for specific proteins or antigens in a sample. The technique was first published by Albert Coons in 1941 to detect the presence of pneumococcal antigen in tissue sections from mice infected with Pneumococcus. Immunocytochemistry helps localization of proteins or antigens in individual cells like blood cells, stem cells, etc., while immunohistochemistry does the same for tissue samples.
These...
Enzyme-Linked Immunosorbent Assay01:33

Enzyme-Linked Immunosorbent Assay

In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen.  Enzyme-substrate reaction allows the antigen to be visualized or quantified.
Immunogold Electron Microscopy01:20

Immunogold Electron Microscopy

Immunoelectron microscopy utilizes immunogold labeling of endogenous proteins with specific antibodies to detect and localize these proteins in cells and tissues. The procedure provides insights into the distribution and quantification of protein under different stimulation conditions offering clues about their functions. Conjugating highly electron-dense gold particles with primary or secondary antibodies allow antigen detection on and within cells, with high resolution and specificity.

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Enzyme-antienzyme method for immunohistochemistry.

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Related Experiment Video

Updated: May 24, 2026

Indirect Immunofluorescence on Frozen Sections of Mouse Mammary Gland
11:13

Indirect Immunofluorescence on Frozen Sections of Mouse Mammary Gland

Published on: December 1, 2015

Enzyme-antienzyme method for immunohistochemistry.

M G Ormerod1, S F Imrie

  • 1The Haddow Laboratories, Institute of Cancer Research, Sutton, Surrey, UK.

Methods in Molecular Biology (Clifton, N.J.)
|February 22, 2012
PubMed
Summary
This summary is machine-generated.

Immunohistochemical stains utilize enzyme-labeled antibodies to visualize specific tissue components. This method allows for nuclear counterstaining and long-term slide storage due to the stain

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Last Updated: May 24, 2026

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Published on: December 1, 2015

Immunohistochemical Analysis in the Rat Central Nervous System and Peripheral Lymph Node Tissue Sections
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Area of Science:

  • Histopathology and Immunohistochemistry

Background:

  • Immunohistochemical (IHC) staining is a crucial technique in pathology.
  • It relies on antibodies to detect specific antigens within tissue samples.

Purpose of the Study:

  • To explain the principles and advantages of enzyme-labeled immunohistochemical stains.

Main Methods:

  • Describes the use of enzyme-labeled antibodies reacting with substrates to produce a colored product.
  • Mentions fluorescent labels as an alternative detection method.

Main Results:

  • Enzyme labels allow for nuclear counterstaining, enhancing visualization of tissue architecture.
  • Stains produced by enzyme labels exhibit slow fading, enabling long-term slide archival.

Conclusions:

  • Enzyme-labeled immunohistochemistry offers superior visualization of tissue architecture.
  • The stability of enzyme-based stains facilitates the preservation of histological slides for future reference.