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Published on: May 17, 2024
[Construction of expression vector from different transcripts of RHD gene].
Jun-Jie Xie1, Yan-Ling Ying, Xian-Guo Xu
1Dalian Medical University Postgraduate School, Dalian, Liaoning Province, China.
Zhongguo Shi Yan Xue Ye Xue Za Zhi
|March 7, 2012
Summary
Researchers constructed expression vectors for RHD gene transcripts, including normal mRNA, DEL9, and DEL89. This work provides a foundation for studying Rh(D) antigen membranous protein expression.
Area of Science:
- Molecular Biology
- Genetics
Context:
- The RHD gene exhibits various alternative transcripts.
- Understanding these transcripts is crucial for RhD antigen expression studies.
Purpose:
- To construct expression vectors for normal RHD mRNA, DEL9, and DEL89 transcripts.
- To facilitate further research into Rh(D) antigen membranous protein expression.
Summary:
- Total RNA was extracted from Rh(D) positive umbilical cord blood.
- RT-PCR was used to obtain RhD cDNA, DEL9, and DEL89 transcripts.
- Sequencing confirmed the correct construction and direction of the three expression vectors.
Impact:
- Successfully constructed expression vectors for distinct RHD gene transcripts.
- Establishes a basis for investigating Rh(D) antigen's membranous protein expression.
- Contributes to the understanding of RHD gene variability and its functional implications.
