Bulk- and surface-modified combinable PDMS capillary sensor array as an easy-to-use sensing device with enhanced...

Area of Science:

• Materials Science
• Analytical Chemistry
• Biomedical Engineering

Background:

• Poly(dimethylsiloxane) (PDMS) is widely used in microfluidic devices.
• Enhancing sensitivity and sample introduction in PDMS-based sensors remains a challenge.
• Multiplex detection of serum analytes requires sensitive and user-friendly platforms.

Purpose of the Study:

• To develop a modified PDMS capillary (CPC) sensor array with enhanced sensitivity and ease of sample introduction.
• To create a
• black
• PDMS material for improved fluorescence detection.
• To demonstrate multiplex detection of serum components using the developed CPC sensor array.

Main Methods:

• Bulk modification of PDMS with India ink.
• Surface modification of PDMS with a silver layer and self-assembled monolayer (SAM) using cysteine.
• Fabrication of a CPC sensor array for simultaneous detection of glucose, potassium, and alkaline phosphatase (ALP).

Main Results:

• Modified PDMS exhibited enhanced fluorescence signals due to surface reflection and reduced background interference.
• Decreased water contact angle facilitated easier sample introduction.
• The CPC sensor array successfully quantified glucose, potassium, and ALP in serum samples.
• The array identified serum components with elevated concentrations.

Conclusions:

• The developed
• black
• PDMS CPC sensor array offers enhanced sensitivity and improved sample handling.
• The sensor platform enables effective multiplex detection of clinically relevant serum analytes.
• This approach holds promise for sensitive and convenient diagnostic applications.