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Making immortalized cell lines from embryonic mouse kidney.

Guanping Tai1, Peter Hohenstein, Jamie A Davies

  • 1University of Edinburgh, Hugh Robson Building, Edinburgh, UK.

Methods in Molecular Biology (Clifton, N.J.)
|May 29, 2012
PubMed
Summary
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Researchers developed a method to create immortalized mouse kidney cell lines for studying renal development and screening drugs. This approach reduces the need for animal testing in kidney research.

Area of Science:

  • Nephrology
  • Developmental Biology
  • Cell Biology

Background:

  • Embryonic mouse kidney cell lines are valuable for studying renal development and disease.
  • Current methods may require significant animal resources for drug screening.
  • The Immortomouse offers a unique genetic background for generating conditionally immortalized cells.

Purpose of the Study:

  • To present a simple method for generating immortalized embryonic mouse kidney cell lines.
  • To facilitate research into renal development, signaling pathways, and gene function.
  • To enable efficient screening of small molecules for treating kidney pathologies like cystogenesis.

Main Methods:

  • Utilizing the Immortomouse, which contains a temperature-sensitive SV40 large T-antigen.

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  • Employing an interferon-regulated promoter to control the expression of the SV40 antigen.
  • Establishing a protocol for generating and maintaining these conditionally immortalized kidney cell lines.

    • Main Results:

    • Successful generation of immortalized embryonic mouse kidney cell lines.
    • Demonstrated utility of these cell lines for studying renal development.
    • Validated the potential for using these lines in high-throughput screening of therapeutic compounds.

    Conclusions:

    • The described method provides a straightforward way to create valuable tools for kidney research.
    • Conditionally immortalized mouse kidney cell lines can accelerate the discovery of treatments for renal diseases.
    • This technique supports the principles of the 3Rs (Replacement, Reduction, Refinement) in animal research.