Glicemical analysis of human blood serum using FT-Raman: a new approach

Fourier transform Raman spectroscopy (FT-Raman) is a noninvasive diagnostic tool largely applied to the analysis of biological fluids and tissues.

We examined the variation of carbohydrate concentration in human blood during 180 min relative to 29 spectra of patients split into four categories: hypoglycemic, healthy, threshold, and diabetic. The main bands monitored were placed in 960, 1030, 1091, 1128, and 1205 cm(-1). These bands were respectively attributed to C-O, C-C (stretching), C-O-H and C-O-C of carbohydrates. In this study, the Raman scattering signal of the all the blood samples was collected during 360 sec. The calculated correlation coefficient (R) between the concentration of carbohydrates and the Raman intensity was 0.923.

The obtained results are reasonable according to classical biochemical analysis. Our proposed FT-Raman-based method was shown to be suitable for the monitoring of carbohydrate concentration in human blood, and presented some advantages over classical biochemical methods, such as real-time analysis, required small sample volume, and was nondestructive, and the samples did not need any previous treatment.