Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Swept-Source OCT Biometry-Derived Corneal Optical Quality: Repeatability Limits for IOL Design Selection in Refractive Cataract Surgery.

Journal of refractive surgery (Thorofare, N.J. : 1995)·2026
Same author

First-Line Faricimab in Diabetic Macular Edema: Insights from a Real-World Treatment-Naïve Population in Austria.

Journal of clinical medicine·2026
Same author

AI-driven digital holographic microscopy for label-free quantitative cellular analysis: toward low-cost and field-deployable platforms.

Biomedical optics express·2026
Same author

Morphological investigation of astrocyte brain cells using quantitative phase imaging.

Biomedical optics express·2026
Same author

Retinal Characteristics in Eyes With Retinal Vein Occlusion Using Widefield Swept-Source Optical Coherence Tomography Angiography.

Investigative ophthalmology & visual science·2026
Same author

Security authentication and tracking of unmanned moving vehicles with optical ID tags.

Optics express·2026
Same journal

Gaussian-modulated continuous-variable quantum key distribution over 60 km fiber using an integrated silicon photonic receiver.

Optics letters·2026
Same journal

E2E-OCT: end-to-end joint learning model using optical coherence tomography images for vocal cord leukoplakia diagnosis.

Optics letters·2026
Same journal

Holographic generation of panoramic 3D scenes by concave ellipsoidal mirror reflection.

Optics letters·2026
Same journal

Dual-pilot phase recovery with pair-wise maximum-ratio combining for coherent PONs.

Optics letters·2026
Same journal

Mapping the whispering gallery modes of a CaF<sub>2</sub> disk resonator with half-tapered fibers to estimate the fundamental mode volume.

Optics letters·2026
Same journal

Quantitative estimation of deep-subwavelength scale via dark-field scattering axial energy concentration decay profiles.

Optics letters·2026
See all related articles

Related Experiment Video

Updated: May 21, 2026

Digital Inline Holographic Microscopy (DIHM) of Weakly-scattering Subjects
10:16

Digital Inline Holographic Microscopy (DIHM) of Weakly-scattering Subjects

Published on: February 8, 2014

In-line reference-delayed digital holography using a low-coherence light source.

Amardeep S G Singh1, Tilman Schmoll, Bahram Javidi

  • 1Center of Medical Physics and Biomedical Engineering, Medical University of Vienna, Währinger Gürtel 18–20, 4L, 1090 Vienna, Austria.

Optics Letters
|June 30, 2012
PubMed
Summary
This summary is machine-generated.

We developed a simple holographic imaging device using low-coherence light for microscopy and in vivo ophthalmic imaging. The system achieves diffraction-limited resolution and allows for aberration correction, demonstrating its potential for advanced optical applications.

More Related Videos

Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization
10:28

Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization

Published on: July 5, 2016

Lensfree On-chip Tomographic Microscopy Employing Multi-angle Illumination and Pixel Super-resolution
08:41

Lensfree On-chip Tomographic Microscopy Employing Multi-angle Illumination and Pixel Super-resolution

Published on: August 16, 2012

Related Experiment Videos

Last Updated: May 21, 2026

Digital Inline Holographic Microscopy (DIHM) of Weakly-scattering Subjects
10:16

Digital Inline Holographic Microscopy (DIHM) of Weakly-scattering Subjects

Published on: February 8, 2014

Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization
10:28

Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization

Published on: July 5, 2016

Lensfree On-chip Tomographic Microscopy Employing Multi-angle Illumination and Pixel Super-resolution
08:41

Lensfree On-chip Tomographic Microscopy Employing Multi-angle Illumination and Pixel Super-resolution

Published on: August 16, 2012

Area of Science:

  • Optical Engineering
  • Biomedical Imaging
  • Holography

Background:

  • Traditional holographic microscopy setups can be complex.
  • Aberration correction is crucial for high-resolution imaging.
  • In vivo ophthalmic imaging requires non-invasive, high-resolution techniques.

Purpose of the Study:

  • To present a simplified holographic imaging device.
  • To explore its potential for microscopy and in vivo ophthalmic imaging.
  • To demonstrate diffraction-limited resolution and aberration correction capabilities.

Main Methods:

  • Utilized a low-coherence light source.
  • Employed the objective lens reflection as reference illumination.
  • Performed in vitro experiments with a resolution test target.
  • Quantified system performance and demonstrated aberration correction.

Main Results:

  • Achieved diffraction-limited resolution.
  • Successfully demonstrated aberration correction.
  • Presented preliminary results with a scattering sample.
  • Validated the system's simplicity and adaptability.

Conclusions:

  • The developed holographic imager offers a simplified and effective approach.
  • It shows significant promise for advanced microscopy and in vivo ophthalmic applications.
  • The system's ability to correct aberrations enhances its utility.