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Updated: May 16, 2026

Drug-induced Sensitization of Adenylyl Cyclase: Assay Streamlining and Miniaturization for Small Molecule and siRNA Screening Applications
Published on: January 27, 2014
[Establishment of a high throughput screening model of cannabinoid receptor 2 agonist]
Yuan Liu1, Zhi Dong, Xiao-qiu Xiao
1Department of Pharmacology, Chongqing Medical University, Chongqing, China.
Aim:
To establish a drug screening model of CB(2) agonist in vitro based on signal regulation pathway.
Methods:
Plasmid pIRES(2)-EGFP-CB(2), pGL(4), 29[luc2P/CRE/Hygro] and PRL-TK were co-transfected into CHO cells in 96 wells plate, to screen agonists of CB(2) receptor by detecting the expressing levels of dual luciferase activity. The concentration and acting time of the agonist were optimized and the stability of the model were investigated.
Results:
The largest relative induction activity was obtained after 8h drug administration. Establishment of a high throughput screening model for CB(2) receptor agonist. The Z' factor is 0.75 demonstrating its perfect stability.
Conclusion:
Successfully establish a drug screening model of CB(2) agonist, which provided a basis for searching valid material from traditional Chinese medicine.
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