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Updated: May 16, 2026

Live Cell Imaging of F-actin Dynamics via Fluorescent Speckle Microscopy (FSM)
Published on: August 5, 2009
Reduction of speckle noise in multiwavelength contouring
Claas Falldorf1, Silke Huferath-von Luepke, Christoph von Kopylow
1Bremer Institut für Angewandte Strahltechnik, Klagenfurter Str. 2, Bremen 28359, Germany. falldorf@bias.de
Abstract:
We report on a method that can be used to improve the result of multiwavelength contouring in the case of objects with rough surface. It is based on the combined evaluation of multiple measurements with varying direction of illumination. While the individual measurements share the same systematics with respect to the shape of the investigated object, the noise arising from speckle decorrelation fluctuates statistically and hence can be reduced by means of averaging. For the case of three illumination directions we show that weighted averaging of the measured phase distributions enhances the signal-to-noise ratio by approximately 3 dB.
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