Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Flow Cytometry

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
In...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Rate and Predictors of Urine Culture Positivity Following Single-Dose Antibiotic Administration: a Prospective Single-Center Study.

Infectious diseases and therapy·2025

Same author

Spectrally encoded flow cytometry using few-mode fiber collection.

Biomedical optics express·2025

Same author

Measuring the Acoustic Reflex through the Tympanic Membrane.

Audiology & neuro-otology·2024

Same author

Pinhole shifting for reducing speckle contrast in reflectance confocal microscopy.

Optics letters·2023

Same author

In vivo optical mapping of the tympanic membrane impulse response.

Hearing research·2023

Same author

Measuring the red blood cell shape in capillary flow using spectrally encoded flow cytometry.

Biomedical optics express·2022

Same journal

Gaussian-modulated continuous-variable quantum key distribution over 60 km fiber using an integrated silicon photonic receiver.

Optics letters·2026

Same journal

E2E-OCT: end-to-end joint learning model using optical coherence tomography images for vocal cord leukoplakia diagnosis.

Optics letters·2026

Same journal

Holographic generation of panoramic 3D scenes by concave ellipsoidal mirror reflection.

Optics letters·2026

Same journal

Dual-pilot phase recovery with pair-wise maximum-ratio combining for coherent PONs.

Optics letters·2026

Same journal

Mapping the whispering gallery modes of a CaF<sub>2</sub> disk resonator with half-tapered fibers to estimate the fundamental mode volume.

Optics letters·2026

Same journal

Quantitative estimation of deep-subwavelength scale via dark-field scattering axial energy concentration decay profiles.

Optics letters·2026

See all related articles

Search research articles

Related Experiment Video

Updated: May 15, 2026

Analysis of Cell Suspensions Isolated from Solid Tissues by Spectral Flow Cytometry

Analysis of Cell Suspensions Isolated from Solid Tissues by Spectral Flow Cytometry

Published on: May 5, 2017

High-speed interferometric spectrally encoded flow cytometry.

Lior Golan¹, Daniella Yeheskely-Hayon, Limor Minai

¹Faculty of Biomedical Engineering, Technion—Israel Institute of Technology, Haifa 32000, Israel.

|December 22, 2012

Summary

This summary is machine-generated.

A new spectrally encoded flow cytometry (SEFC) system enables label-free, in vivo imaging of blood cells. This advanced technique allows for faster, more accurate diagnosis and noninvasive monitoring of blood in patients.

More Related Videos

Microfluidic Imaging Flow Cytometry by Asymmetric-detection Time-stretch Optical Microscopy (ATOM)

Microfluidic Imaging Flow Cytometry by Asymmetric-detection Time-stretch Optical Microscopy (ATOM)

Published on: June 28, 2017

Cortical Actin Flow in T Cells Quantified by Spatio-temporal Image Correlation Spectroscopy of Structured Illumination Microscopy Data

Cortical Actin Flow in T Cells Quantified by Spatio-temporal Image Correlation Spectroscopy of Structured Illumination Microscopy Data

Published on: December 17, 2015

Related Experiment Videos

Last Updated: May 15, 2026

Analysis of Cell Suspensions Isolated from Solid Tissues by Spectral Flow Cytometry

Analysis of Cell Suspensions Isolated from Solid Tissues by Spectral Flow Cytometry

Published on: May 5, 2017

Microfluidic Imaging Flow Cytometry by Asymmetric-detection Time-stretch Optical Microscopy (ATOM)

Microfluidic Imaging Flow Cytometry by Asymmetric-detection Time-stretch Optical Microscopy (ATOM)

Published on: June 28, 2017

Cortical Actin Flow in T Cells Quantified by Spatio-temporal Image Correlation Spectroscopy of Structured Illumination Microscopy Data

Cortical Actin Flow in T Cells Quantified by Spatio-temporal Image Correlation Spectroscopy of Structured Illumination Microscopy Data

Published on: December 17, 2015

Area of Science:

Biomedical optics
Medical imaging
Flow cytometry

Background:

Spectrally encoded flow cytometry (SEFC) offers potential for noninvasive in vivo microscopy of blood cells.
Current limitations exist in imaging speed and depth for flowing blood cells.

Purpose of the Study:

Introduce a novel SEFC system for label-free confocal imaging of blood cells.
Enhance the capability to image rapidly flowing blood cells in vivo.

Main Methods:

Development of a novel SEFC system utilizing interferometric Fourier-domain detection.
Incorporation of a high-speed wavelength-swept source enabling a 100 kHz line rate.
Imaging of blood cells flowing at velocities up to 10 mm/s within 65 μm-diameter vessels at depths of 80 μm below the tissue surface.

Main Results:

Successful label-free confocal imaging of blood cells in vivo.
Achieved high-speed imaging of rapidly flowing blood cells.
Demonstrated capability to image cells at a depth of 80 μm within small vessels.

Conclusions:

The novel SEFC system provides high-speed, label-free imaging of flowing blood cells.
This technique has the potential to improve diagnostic accuracy and reduce imaging time.
Opens new avenues for noninvasive monitoring of blood in clinical settings.