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Vortex Counter-Current Chromatography: Performance of a New Preparative Column
Yoichiro Ito1, Robert Clary, Jacob J Witten
1Bioseparation Technology Laboratory, Biochemistry and Biophysics Center, National Heart, Lung, and Blood Institute, National Institutes of Health, Bldg. 10, Room 8N230, 10 Center Drive, Bethesda, MD 20892-1762, USA.
Chromatographia
|March 19, 2013
Summary
A new vortex counter-current chromatograph column was developed, achieving high partition efficiency for separating DNP-amino acids, dipeptides, and Sudan dyes. Further improvements in column configuration may enhance performance.
Area of Science:
- Analytical Chemistry
- Chromatography
Background:
- Counter-current chromatography (CCC) is a liquid-liquid separation technique.
- Vortex-based CCC offers potential for enhanced separation efficiency.
Purpose of the Study:
- To fabricate and evaluate a new preparative column for vortex counter-current chromatography (VCCC).
- To assess the separation performance of the VCCC column using diverse solvent systems and analytes.
Main Methods:
- Fabrication of a VCCC column with 966 cylindrical separation units on a polyethylene disk.
- Testing the column with three distinct two-phase solvent systems.
- Separation of DNP-amino acids, dipeptides, and Sudan dyes.
Main Results:
- The fabricated column has a total capacity of 364 mL.
- High partition efficiency, exceeding a thousand theoretical plates, was observed for most separations.
- Successful separation of DNP-amino acids, dipeptides, and Sudan dyes was achieved.
Conclusions:
- The developed VCCC column demonstrates high separation efficiency.
- Column configuration modifications hold potential for further performance enhancement.
- This preparative VCCC column is suitable for various separation applications.
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