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Published on: September 23, 2022
[Detection of weak D antigen by flow cytometry]
Xiao-Ying Wu1, Hong-Xian Xu, Wen Xiong
1Shenzhen Blood Center, Shenzhen, Guangdong Province, China.
Zhongguo Shi Yan Xue Ye Xue Za Zhi
|May 1, 2013
Summary
Flow cytometry can detect weak D and partial D phenotypes, but is not sensitive enough for DEL variant detection. Its Rh(D) antigen detection sensitivity is comparable to the indirect antiglobulin test (IAT).
Area of Science:
- Immunology
- Hematology
- Genetics
Context:
- Flow cytometry is a potential tool for analyzing Rh(D) antigen density.
- Previous studies suggested its use for routine weak D positive phenotype detection.
Purpose:
- To evaluate the practicability of flow cytometry for detecting weak D positive phenotypes.
- To compare flow cytometry's sensitivity with saline, IAT, and absorption/elution tests for Rh(D) variants.
Summary:
- Six weak D positive, 7 DEL individuals, and controls were tested using flow cytometry.
- Weak D type 15 and partial D type DVI samples were detected as positive by flow cytometry (P < 0.05).
- Seven DEL samples tested negative (P > 0.05), indicating flow cytometry's insufficient sensitivity for DEL detection.
Impact:
- Flow cytometry sensitivity for D antigen detection is similar to IAT but lower than absorption/elution.
- IAT is more practical than flow cytometry for weak D/partial D detection.
- Flow cytometry is not sensitive enough for identifying DEL variants.
