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Updated: May 10, 2026
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Sample Preparation for Endopeptidomic Analysis in Human Cerebrospinal Fluid
Published on: December 4, 2017
An automated RP-SCX solid-phase extraction procedure for urinary peptidomics biomarker discovery studies
Crina I A Balog1, Rico Derks, Oleg A Mayboroda
1Department of Parasitology, Leiden University Medical Center, RC Leiden, The Netherlands.
Methods in Molecular Biology (Clifton, N.J.)
|June 15, 2013
Summary
This study introduces a fast and reliable method for screening urine samples to discover new disease biomarkers. The technique efficiently extracts native peptides for improved disease diagnosis and monitoring.
Area of Science:
- Clinical chemistry
- Biomarker discovery
- Proteomics
Background:
- Urine analysis is crucial for disease diagnosis, monitoring treatment, and prognosis due to its accessibility.
- Current methods for identifying urine biomarkers are often laborious, involving multiple purification and separation steps.
Purpose of the Study:
- To develop a generally applicable, rapid, and robust method for screening numerous urine samples.
- To enable the discovery of native peptides as potential biomarkers.
Main Methods:
- The new method combines online sample pretreatment with mass spectrometry.
- Native peptides are extracted from urine using a miniaturized reverse-phase-strong cation exchange cartridge system.
- The procedure is designed to yield sufficient material for identifying and characterizing differentially expressed markers using multiple mass analyzers.
Main Results:
- The developed method allows for the screening of a large number of urine samples.
- It yields a broad spectrum of native peptides, facilitating biomarker discovery.
- The integrated approach simplifies the process compared to traditional multi-step purification.
Conclusions:
- This rapid and robust method offers a powerful tool for urine biomarker discovery.
- It streamlines the analysis of native peptides, aiding in disease diagnosis and monitoring.
- The technique supports the identification of differentially expressed markers for clinical applications.
