Related Experiment Video
Updated: May 9, 2026

Single Molecule Fluorescence Microscopy on Planar Supported Bilayers
Published on: October 31, 2015
Camera technologies for low light imaging: overview and relative advantages.
1Hamamatsu Photonic Systems, Division of Hamamatsu Corporation, Bridgewater, New Jersey, USA.
This review compares four camera technologies for low light imaging: cooled charge-coupled device (CCD), cooled scientific complementary metal-oxide-semiconductor (sCMOS), intensified, and electron multiplier cameras. It highlights practical considerations and noise characteristics for optimal camera selection.
Area of Science:
- Imaging technology
- Optical physics
- Scientific instrumentation
Background:
- Low light imaging presents significant challenges due to signal scarcity and inherent noise.
- Selecting appropriate camera technology is crucial for effective data acquisition in low light conditions.
Purpose of the Study:
- To provide a comprehensive overview of current camera technologies for low light imaging.
- To compare practical aspects and performance characteristics of different camera types.
- To elucidate the impact of noise on low light imaging performance.
Main Methods:
- Comparative analysis of four major camera technologies: cooled CCD, cooled sCMOS, intensified cameras, and electron multiplier cameras.
- Discussion of photoelectron and camera noise characteristics.
- Emphasis on practical considerations for each camera technology.
Main Results:
- Detailed comparison of the advantages and disadvantages of each camera technology for low light applications.
- Explanation of how noise sources affect image quality and data interpretation.
- Insights into the practical implementation and use of these cameras.
Conclusions:
- The choice of camera technology significantly impacts low light imaging capabilities.
- Understanding noise characteristics is essential for optimizing camera performance.
- This review aids researchers in selecting the most suitable camera for their specific low light imaging needs.
More Related Videos
Related Concept Videos
Three-Dimensional Microscopy in Microbiology
Overview of Microscopy Techniques
Imaging Biological Samples with Optical Microscopy
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
Two-Dimensional Microscopy in Microbiology
Confocal Fluorescence Microscopy
Phase Contrast and Differential Interference Contrast Microscopy
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...

