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Quantitative Measure of Lung Structure and Function Obtained from Hyperpolarized Xenon Spectroscopy
Published on: November 10, 2023
Validating excised rodent lungs for functional hyperpolarized xenon-129 MRI.
David M L Lilburn1, Theodore Hughes-Riley, Joseph S Six
1Sir Peter Mansfield Magnetic Resonance Centre, School of Clinical Sciences, University of Nottingham, Nottingham, United Kingdom.
Plos One
|September 12, 2013
Summary
Ex vivo rodent lung models with hyperpolarized (129)Xe MRI offer simplified physiological measurements of respiratory function. This method provides valuable insights into lung function and airway responsiveness, aiding drug development.
Area of Science:
- Pulmonary physiology
- Medical imaging
- Respiratory research
Background:
- In vivo MRI for lung function is technically challenging.
- Ex vivo models offer a simplified approach for physiological measurements.
Purpose of the Study:
- To explore ex vivo rodent lung models for physiological measurements using hyperpolarized (129)Xe MRI.
- To demonstrate the feasibility of obtaining detailed lung function data without in vivo constraints.
Main Methods:
- Utilized ex vivo rodent lung models with a custom breathing apparatus.
- Employed hyperpolarized (129)Xe MRI to image gas distribution and lung volumes.
- Administered methacholine challenges to assess airway responsiveness post mortem.
Main Results:
- Achieved spatially resolved imaging of inhaled hyperpolarized (129)Xe gas distribution.
- Obtained residual lung volume (RV) data using straightforward MRI protocols.
- Demonstrated persistent post mortem airway responsiveness to methacholine.
Conclusions:
- Ex vivo lung models with hyperpolarized (129)Xe MRI simplify technical challenges and offer unique physiological insights.
- This methodology facilitates lung function studies in health and disease, aiding drug development.
- The approach augments histological data and bypasses regulatory requirements for in vivo studies.
