Related Experiment Video
Updated: Apr 30, 2026

A Sample Preparation Pipeline for Microcrystals at the VMXm Beamline
Published on: June 17, 2021
Macromolecular crystallography beamline X25 at the NSLS
Annie Héroux1, Marc Allaire1, Richard Buono1
1Photon Sciences Directorate, Brookhaven National Laboratory, PO Box 5000, Upton, NY 11973-5000, USA.
Abstract:
Beamline X25 at the NSLS is one of the five beamlines dedicated to macromolecular crystallography operated by the Brookhaven National Laboratory Macromolecular Crystallography Research Resource group. This mini-gap insertion-device beamline has seen constant upgrades for the last seven years in order to achieve mini-beam capability down to 20 µm × 20 µm. All major components beginning with the radiation source, and continuing along the beamline and its experimental hutch, have changed to produce a state-of-the-art facility for the scientific community.
Related Concept Videos
X-ray Crystallography
Diffraction
Diffraction is the change in the direction of travel experienced by an electromagnetic wave when it encounters a physical barrier whose dimensions are comparable to those of the wavelength of the light. X-rays are electromagnetic radiation with wavelengths about as long as the distance between neighboring...
X-ray Diffraction of Biological Samples
According to Bragg's law, when X-rays strike the sample positioned on a stage, the rays are scattered by the electron clouds around the sample atoms. The X-ray diffraction or scattering is caused by constructive interference of the X-ray waves that reflect off the internal...
Determination of Crystal Structures

