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Updated: Apr 20, 2026

Modified MicroSecure Vitrification: A Safe, Simple and Highly Effective Cryopreservation Procedure for Human Blastocysts
Published on: March 2, 2017
[Effects of artificial shrinkage prior to vitrification in a closed system: a randomized controlled trial]
A Gala1, A Ferrières2, S Assou3
1Institut de recherche en biothérapie, hôpital Saint-Éloi, CHU de Montpellier, 34000 Montpellier, France; U1040, Inserm, 34000 Montpellier, France; UFR de médecine, université Montpellier-1, 34000 Montpellier, France; Service de gynécologie-obstétrique, hôpital Arnaud-de-Villeneuve, CHU de Montpellier, 34295 Montpellier cedex 5, France.
Objective:
To evaluate the effect of induced blastocoele shrinkage before vitrification in a closed carrier device.
Patients And Methods:
Prior to vitrification, blastocyst cavity was artificially shrinked by laser pulse or not treated according to a 2:1 randomized procedure. A total of 185 warming cycles from April 2011 to March 2013 have been analyzed. Clinical pregnancy rate and survival rate were compared between the two groups. The mean (±SD) women age was 33.5±5.7 years for both groups.
Results:
The pregnancy rate in the group with artificial reduction of the cavity was higher ([32/67] 47.7%) than in the control group but not significantly ([43/113] 38%). The survival rate in the artificial shrinkage group was significantly higher compared with the control group : 99% (102/103) and 91.8% (168/183) respectively (P=0.01).
Discussion And Conclusion:
This study reveals that artificial shrinkage of blastocoelic cavity by laser pulse before vitrification in a closed carrier device improves survival rate after warming.
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