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Prototyping the Experimental Setup to Quantify the Tissue Oxygen Consumption Rate and Its Preliminary Test.
N Watanabe1,2, M Shibata3,4, S Sawada4
1Department of Bioscience and Engineering, College of Systems Engineering and Science, Shibaura Institute of Technology, 307 Fukasaku, Minuma-Ku, Saitama, 337-8570, Japan. nobuo@sic.shibaura-it.ac.jp.
Advances in Experimental Medicine and Biology
|January 20, 2016
Summary
We developed a novel method to assess tissue viability by measuring oxygen consumption rate. Fresher tissues demonstrated higher oxygen transfer, indicating this method can reliably evaluate tissue health.
Area of Science:
- Biomedical Engineering
- Physiology
- Tissue Viability Assessment
Background:
- Establishing reliable methods for diagnosing amputated extremity viability is crucial.
- Current diagnostic methods may lack practicality or reliability for immediate assessment.
Purpose of the Study:
- To propose and validate a novel method for assessing tissue viability using oxygen consumption rate.
- To examine the feasibility of a prototyped experimental system for measuring tissue oxygen transfer.
Main Methods:
- Developed an experimental system to assess oxygen transfer rate into tissue.
- Measured the rate of decrease in dissolved oxygen (DO) concentration in surrounding buffer fluid.
- Compared oxygen consumption between fresh and non-fresh rat skeletal muscles.
Main Results:
- Fresher rat skeletal muscles exhibited a higher rate of oxygen transfer into the tissue.
- Demonstrated a correlation between tissue oxygen consumption and tissue freshness.
- Indicated that oxygen consumption rate can serve as an indirect measure of tissue viability.
Conclusions:
- The prototyped experimental system is feasible for assessing tissue oxygen consumption.
- Tissue oxygen consumption rate is a reliable indicator of tissue freshness and viability.
- This method offers a practical approach for diagnosing the viability of amputated extremities.
