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Updated: Mar 18, 2026

In Vitro Assay to Measure Phosphatidylethanolamine Methyltransferase Activity
Published on: January 5, 2016
A calcium-dependent acyltransferase that produces N-acyl phosphatidylethanolamines
Yuji Ogura1,2, William H Parsons1,2, Siddhesh S Kamat1,2
1Department of Chemical Physiology, The Scripps Research Institute, La Jolla, California, USA.
Abstract:
More than 30 years ago, a calcium-dependent enzyme activity was described that generates N-acyl phosphatidylethanolamines (NAPEs), which are precursors for N-acyl ethanolamine (NAE) lipid transmitters, including the endocannabinoid anandamide. The identity of this calcium-dependent N-acyltransferase (Ca-NAT) has remained mysterious. Here, we use activity-based protein profiling to identify the poorly characterized serine hydrolase PLA2G4E as a mouse brain Ca-NAT and show that this enzyme generates NAPEs and NAEs in mammalian cells.
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