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An automated optoelectronic reticulocyte counter
K Kojima1, M Niri, K Setoguchi
1Department of Clinical and Laboratory Medicine, Osaka City, University Medical School, Japan.
American Journal of Clinical Pathology
|July 1, 1989
Summary
Automated reticulocyte counting using flow cytometry offers a precise and rapid alternative to manual methods. This new system accurately measures reticulocyte counts and maturity, aiding in anemia diagnosis and red blood cell kinetics studies.
Area of Science:
- Hematology
- Clinical Laboratory Science
- Flow Cytometry
Background:
- Manual reticulocyte counting is laborious and prone to inaccuracies.
- There is a need for automated, reliable methods for reticulocyte assessment.
Purpose of the Study:
- To evaluate the utility of a new automated reticulocyte counter (Sysmex R-1000) for laboratory use.
- To assess the correlation between automated and manual reticulocyte counts.
- To determine if the automated system can differentiate reticulocyte maturity.
Main Methods:
- Utilized the Sysmex R-1000, a flow cytometer employing auramine O staining and argon-laser excitation.
- Compared automated counts with manual microscopic counts.
- Analyzed reticulocyte maturity based on fluorescence intensity (high, moderate, slight).
Main Results:
- High correlation (r = 0.941) between automated and manual reticulocyte counts.
- Demonstrated high linearity and reproducibility.
- Successfully differentiated reticulocyte maturity, with distinct patterns observed in various patient groups (chemotherapy, hemolytic anemia, renal failure).
Conclusions:
- Automated reticulocyte counting provides reliable and efficient data.
- The Sysmex R-1000 is a valuable tool for red blood cell kinetics analysis and anemia pathogenesis studies.
- Automated analysis of reticulocyte maturity can aid in diagnosing underlying conditions.