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Related Concept Videos

Cell Culture01:21

Cell Culture

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Most vertebrate cells grow in vitro attached to a substrate as a monolayer, called adherent cultures. The flasks and plates used to grow cells are chemically treated to facilitate cell attachment. However, a few cell types, such as hematopoietic cells, can grow in a suspension. In contrast to adherent cultures, suspension cultures can grow in non-treated cultureware using magnetic stirrers or spinner flasks to agitate the culture media
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Stem cell research aims to find ways to use stem cells to regenerate and repair cellular damage. Over time, most adult cells undergo the wear and tear of aging and lose their ability to divide and repair themselves. Stem cells do not display a particular morphology or function. Adult stem cells, which exist as a small subset of cells in most tissues, keep dividing and can differentiate into a number of specialized cells generally formed by that tissue. These cells enable the body to renew and...
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A cell line is a population of cells grown in vitro that can be subcultured over several generations. Normal cells cease to divide after a certain number of cell divisions, a process known as replicative senescence. This number, called the Hayflick limit, was conceptualized by Leonard Hayflick in 1961 when he observed that fetal cells grown in culture could only divide 40-60 times. This limit is due to the shortening of the telomeres during each round of cell division, preventing cell division...
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Related Experiment Video

Updated: Dec 10, 2025

A Cell Culture Model for Producing High Titer Hepatitis E Virus Stocks
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A Cell Culture Model for Producing High Titer Hepatitis E Virus Stocks

Published on: June 26, 2020

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HEV Cell Culture.

Feng Zhang1, Youchun Wang2

  • 1Division of Monoclonal Antibody, National Institutes for Food and Drug Control, No. 2 Tiantanxili, Dongcheng District, Beijing, 100050, China.

Advances in Experimental Medicine and Biology
|October 15, 2016
PubMed
Summary
This summary is machine-generated.

Hepatitis E virus (HEV) research advanced with cell culture and infectious cDNA clones. These methods revealed HEV genetic mutations increase virulence and identified pORF3

Keywords:
Cell cultureCytopathetic effectPassageTissue culture infective dose

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Area of Science:

  • Virology
  • Cell Biology

Background:

  • Hepatitis E virus (HEV) culture has been challenging, limiting research.
  • Existing cell culture systems for HEV are limited in efficiency.
  • Understanding HEV replication and virulence factors is crucial.

Purpose of the Study:

  • To explore efficient cell culture systems for HEV.
  • To investigate HEV genetic mutations and their impact on virulence.
  • To utilize infectious cDNA clones for studying HEV properties.

Main Methods:

  • Optimization of virus stocks, host cells, and medium components for HEV culture.
  • Construction and application of infectious HEV cDNA clones.
  • Analysis of viral genetic mutations during cell passage.
  • Study of viral thermal stability, host range, and protein functions.

Main Results:

  • Two efficient HEV cell culture systems were identified.
  • Genetic mutations during HEV passage correlated with increased virulence.
  • Infectious cDNA clones facilitated studies on viral properties.
  • The pORF3 protein was associated with the envelopment of secreted HEV, explaining infection in the presence of antibodies.

Conclusions:

  • Efficient HEV cell culture and infectious cDNA clones are valuable research tools.
  • HEV genetic variability influences virulence in cell culture.
  • pORF3 plays a key role in HEV virion formation and infectivity.