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Updated: Mar 11, 2026
MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as a Novel Detection and Quantification Method
Published on: October 7, 2025
Multiplex miRNA assay using lanthanide-tagged probes and the duplex-specific nuclease amplification strategy
Shixi Zhang1, Rui Liu2, Zhi Xing1
1Beijing Key Laboratory for Microanalytical Methods and Instrumentation, Department of Chemistry, Tsinghua University, Beijing, 100084, China. sczhang@mail.tsinghua.edu.cn.
Abstract:
A multiplex ICP-MS-based miRNA assay with duplex-specific nuclease amplification using bifunctional oligonucleotide probes was proposed. A multiplex assay of miR-141, let-7d, and miR-21 could be achieved simultaneously.
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