Related Experiment Video
Updated: Mar 7, 2026
07:59
Efficient and Consistent Generation of Retinal Pigment Epithelium/Choroid Flatmounts from Human Eyes for Histological Analysis
Published on: October 28, 2022
3.5K
INTERNAL LIMITING MEMBRANE PEELING-DEPENDENT RETINAL STRUCTURAL CHANGES AFTER VITRECTOMY IN RHEGMATOGENOUS RETINAL
Toshio Hisatomi1,2, Takashi Tachibana1, Shoji Notomi1,2
1Department of Ophthalmology, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan.
Retina (Philadelphia, Pa.)
|February 25, 2017
Summary
Three-dimensional optical coherence tomography (3D-OCT) revealed retinal changes after internal limiting membrane (ILM) peeling in rhegmatogenous retinal detachment cases. These changes, including thinning and layer appearance alterations, were evident and persistent for at least two months post-surgery.
Area of Science:
- Ophthalmology
- Retinal Surgery
- Medical Imaging
Background:
- Rhegmatogenous retinal detachment (RRD) is a serious condition requiring surgical intervention.
- Internal limiting membrane (ILM) peeling is a common surgical step during vitrectomy for RRD.
- Understanding the post-operative retinal changes is crucial for patient outcomes.
Purpose of the Study:
- To investigate retinal alterations following vitrectomy with ILM peeling in RRD patients.
- To assess the utility of 3D-OCT in visualizing these specific retinal changes.
Main Methods:
- A study of 68 eyes from 67 patients undergoing vitrectomy for RRD with ILM peeling.
- Brilliant blue G staining was used to aid ILM peeling with fine forceps.
- Three-dimensional optical coherence tomography (3D-OCT) with volume-rendering technology was employed for imaging.
Main Results:
- 3D-OCT identified ILM peeling-related retinal changes in 66% of eyes.
- Observed changes included: dissociated optic nerve fiber layer appearance, dimple sign, and macular thinning.
- Forceps-related retinal thinning was noted in both attached and detached macula cases; no postoperative macular pucker occurred.
Conclusions:
- 3D-OCT effectively visualizes spatial and temporal retinal changes post-ILM peeling.
- These identified retinal changes manifest within two months and are persistent.
