Oxygen Consumption Rate and Energy Expenditure in Mice: Indirect Calorimetry

Global obesity epidemic demands more effective therapeutic treatments and better understanding of obesity pathophysiology. Since obesity results from energy imbalance, accurate quantification of energy intake and energy expenditure (EE) becomes an essential prerequisite to phenotype the cause for obesity development. Indirect calorimetry has long been used as one of the most established methods in EE quantification by detecting changes in levels of O₂ consumption and CO₂ production. In this article, we describe procedures and important considerations for an effective measurement using indirect calorimetry.