Phenotyping Multiple Subsets of Immune Cells In Situ in Formalin-Fixed, Paraffin-Embedded Tissue Sections

Some somatic illnesses such as peripheral tumours can present with psychiatric symptoms. Many of these are characterized by changes in biomarkers related to the inflammation or immune response. Here, we describe a multispectral imaging protocol that can be used to phenotype immune and other cell types through simultaneous imaging of multiple proteins in sections of peripheral solid tumours and other tissues. This approach can also be used to assess the spatial organization of these cells within the tissue.