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Updated: Feb 28, 2026

Exploring m6A and m5C Epitranscriptomes upon Viral Infection: an Example with HIV

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Reading the Epitranscriptome: New Techniques and Perspectives.

Lee E Vandivier¹, Brian D Gregory²

¹University of Pennsylvania, Philadelphia, PA, United States; Cell and Molecular Biology Graduate Program, University of Pennsylvania, Philadelphia, PA, United States.

|June 12, 2017

Summary

This summary is machine-generated.

RNA modifications expand RNA's capabilities, with over 100 known chemical additions. High-throughput sequencing reveals these modifications are abundant, especially in messenger RNA, and play key regulatory roles.

Keywords:

High-throughput annotation of modified ribonucleotides High-throughput sequencing MeRIP-seq Pseudouridine-seq RNA covalent modification RNA modification RNA posttranscriptional modification RNA sequencing

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Area of Science:

Molecular Biology
Genomics
Biochemistry

Background:

Over 100 chemical moieties can covalently modify ribonucleotides.
RNA modifications expand the coding and regulatory potential of RNA.
Modifications are abundant in various RNA types, including messenger RNA (mRNA).

Purpose of the Study:

To review methods for surveying RNA modifications.
To highlight the impact of transcriptome-wide methods on understanding RNA modification regulation.

Main Methods:

Targeted methods for RNA modification analysis.
Global methods for surveying RNA modifications.
High-throughput sequencing for transcriptome-wide modification mapping.

Main Results:

High-throughput sequencing has generated transcriptome-wide RNA modification maps.
RNA modifications are prevalent across different RNA classes.
New regulatory functions of RNA modifications have been discovered.

Conclusions:

Transcriptome-wide methods have significantly advanced the understanding of RNA modification.
RNA modifications are crucial for regulating gene expression.
Further research into modification-mediated regulation is warranted.