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Updated: Feb 27, 2026

A Cell-based Assay to Investigate Non-muscle Myosin II Contractility via the Folded-gastrulation Signaling Pathway in Drosophila S2R+ Cells
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Asymmetrically deployed actomyosin-based contractility generates a boundary between developing leg segments in

Dan Ly1, Erin Resch1, George Ordiway1

  • 1Department of Cell and Developmental Biology, Perelman School of Medicine, the University of Pennsylvania, Philadelphia, PA 19104, United States.

Developmental Biology
|July 11, 2017
PubMed
Summary

Developing tissues establish boundaries using mechanical asymmetry, not just adhesion. This study shows Rho Kinase-dependent tension and Myosin II accumulation at the Drosophila leg epithelium boundary, crucial for maintaining tissue separation.

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