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Disaccharides Determination: A Review of Analytical Methods
Marta Pokrzywnicka1, Robert Koncki1
1a Department of Chemistry , University of Warsaw , Warsaw , Poland.
Critical Reviews in Analytical Chemistry
|October 21, 2017
Summary
Disaccharide analysis is crucial for food, metabolism, and biotechnology. This review covers selective and non-selective methods for determining disaccharides in various samples.
Area of Science:
- Analytical Chemistry
- Food Science
- Biotechnology
Background:
- Disaccharides are vital in dietetics, food profiling, metabolism, and technological processes.
- Traditional methods like polarimetry lack selectivity due to disaccharides' similar chemical properties.
- Accurate disaccharide determination often requires sample pre-separation.
Purpose of the Study:
- To review recent analytical literature on disaccharide determination methods.
- To compare non-selective and selective techniques for disaccharide analysis.
- To highlight methods applicable to real-world samples.
Main Methods:
- Review of chromatographic and electrophoretic techniques.
- Exploration of enzymatic methods for selective disaccharide recognition.
- Discussion of chemometric approaches with spectroscopic data.
Main Results:
- Chromatographic and electrophoretic methods are currently popular for disaccharide determination.
- Enzymatic methods offer selective analyte recognition and simpler detection.
- Advanced chemometrics can enhance spectroscopic analysis of disaccharides.
Conclusions:
- Selective methods, including enzymatic and advanced spectroscopic techniques, are increasingly important for accurate disaccharide analysis.
- The choice of method depends on the sample matrix and analytical goals.
- Further research can optimize these methods for broader applications.
