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Author Spotlight: Optimizing iPSC Differentiation for Efficient Production to Generate Kidney Organoids
Published on: September 1, 2023
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Higher-Order Kidney Organogenesis from Pluripotent Stem Cells
Atsuhiro Taguchi1, Ryuichi Nishinakamura1
1Department of Kidney Development, Institute of Molecular Embryology and Genetics, Kumamoto University, 2-2-1 Honjo, Kumamoto 860-0811, Japan.
Cell Stem Cell
|November 14, 2017
Summary
Researchers developed a new method to create kidney organoids that mimic embryonic development. This technique precisely guides pluripotent stem cells to form complex kidney structures, advancing organoid modeling.
Area of Science:
- Developmental Biology
- Stem Cell Biology
- Regenerative Medicine
Background:
- Organogenesis involves intricate assembly of functional units and progenitor cells.
- Current organoid models struggle to replicate complex tissue architectures from pluripotent stem cells (PSCs).
Purpose of the Study:
- To develop a method for assembling kidney organoids that recapitulate embryonic branching morphogenesis.
- To establish a protocol for differential induction of ureteric bud and nephron progenitor lineages from PSCs.
Main Methods:
- Studied distinct origins and developmental pathways of ureteric bud and nephron progenitors.
- Developed a protocol for differential induction of these lineages from mouse and human PSCs.
- Reassembled induced progenitors to form kidney organoids.
Main Results:
- Successfully assembled kidney organoids mimicking embryonic branching morphogenesis.
- Organoids exhibited inherent embryonic kidney architectures, including progenitor niches and differentiated nephrons.
- A ramified ureteric epithelium interconnected the differentiated nephrons.
Conclusions:
- The selective induction and reassembly strategy is effective for recapitulating organotypic architecture in PSC-derived kidney organoids.
- This approach offers a powerful tool for studying kidney development and disease modeling.
