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Updated: Feb 17, 2026

Conducting Multiple Imaging Modes with One Fluorescence Microscope
Published on: October 28, 2018
A green-light-emitting, spontaneously blinking fluorophore based on intramolecular spirocyclization for dual-colour
Shin-Nosuke Uno1, Mako Kamiya, Akihiko Morozumi
1Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan. uranokun@m.u-tokyo.ac.jp.
Abstract:
We have developed the first green-light-emitting, spontaneously blinking fluorophore (SBF), HEtetTFER. In combination with our near-infrared-light-emitting SBF (HMSiR), HEtetTFER allows dual-colour single-molecule localization microscopy (SMLM) in buffer solution without any additive and without photoactivation.
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