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Optical projection tomography for rapid whole mouse brain imaging.
David Nguyen1, Paul J Marchand1, Arielle L Planchette1
1Laboratoire d'Optique Biomédicale, School of Engineering, École Polytechnique Fédérale de Lausanne, CH-1015 Lausanne, Switzerland.
Biomedical Optics Express
|January 4, 2018
Summary
We developed a fast optical projection tomography (OPT) method for whole mouse brain imaging. This technique visualizes neuroanatomy, vasculature, and Alzheimer's disease pathology with high resolution.
Area of Science:
- Life Sciences
- Biomedical Imaging
- Neuroscience
Background:
- Three-dimensional mesoscopic imaging is crucial for whole-organ studies in life sciences.
- Optical Projection Tomography (OPT) offers a powerful approach for detailed biological imaging.
Purpose of the Study:
- To present a novel OPT method for imaging the intact mouse brain.
- To achieve high-resolution, rapid 3D visualization of brain structures and pathology.
Main Methods:
- Developed an optimized 3-day clearing protocol for intact mouse brains.
- Implemented an OPT system with ~50 µm isotropic resolution and 4-8 minute acquisition time.
- Utilized intrinsic brain autofluorescence and specific fluorescent labels for imaging.
Main Results:
- Successfully imaged the 3D neuroanatomy of the mouse brain.
- Visualized the vascular network and amyloid deposition in 5xFAD mouse models of Alzheimer's disease.
- Demonstrated high-resolution OPT images comparable to histological slices.
Conclusions:
- The presented OPT method enables rapid, high-resolution 3D imaging of the intact mouse brain.
- This technique is valuable for studying neuroanatomy, vascularization, and neurodegenerative diseases like Alzheimer's.
- OPT provides a complementary tool to traditional histology for brain research.
Keywords:
(110.0110) Imaging systems(110.6880) Three-dimensional image acquisition(110.6960) Tomography(170.0170) Medical optics and biotechnology(170.2520) Fluorescence microscopy(180.0180) Microscopy(180.6900) Three-dimensional microscopyMore Related Videos
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