Multiplex Surface Plasmon Resonance Imaging-Based Biosensor for Human Pancreatic Islets Hormones Quantification.

Area of Science:

• Biomedical Engineering
• Endocrinology
• Biosensor Technology

Background:

• Diabetes mellitus is characterized by impaired insulin secretion from pancreatic islets.
• Understanding paracrine interactions, like somatostatin's effect on insulin secretion, is crucial for diabetes research.
• Existing methods lack the ability to simultaneously monitor multiple islet hormones.

Purpose of the Study:

• To develop a multiplex biosensor for simultaneous quantification of insulin, glucagon, and somatostatin.
• To investigate the paracrine effects within pancreatic islets.
• To establish a novel tool for diabetes research and therapeutic development.

Main Methods:

• Utilized multiplex surface plasmon resonance imaging (SPRi).
• Designed an antifouling sensing surface using a mixed self-assembly monolayer (SAM) of CH3O-PEG-SH and 16-mercaptohexadecanoic acid.
• Achieved label-free, simultaneous quantification of three key hormones in a complex matrix.

Main Results:

• The SPRi biosensor successfully quantified insulin, glucagon, and somatostatin simultaneously.
• Achieved limits of detection of 1 nM for insulin, 4 nM for glucagon, and 246 nM for somatostatin.
• Demonstrated a total analysis time of 21 minutes per point, representing a significant advancement in speed and multiplexing capability.

Conclusions:

• This novel biosensor enables the first label-free, multiplex measurement of insulin, glucagon, and somatostatin.
• The technology facilitates a deeper understanding of paracrine signaling in pancreatic islets.
• This tool holds potential for advancing diabetes research and developing new therapeutic interventions.