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Multimodal Quantitative Phase Imaging with Digital Holographic Microscopy Accurately Assesses Intestinal Inflammation and Epithelial Wound Healing
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Near-Common-Path Self-Reference Quantitative Phase Microscopy.

Timothy R Hillman1, Niyom Lue1, Yongjin Sung1

  • 1Laser Biomedical Research Center, George R. Harrison Spectroscopy Laboratory, Massachusetts Institute of Technology, Cambridge, MA 02139 USA.

IEEE Photonics Technology Letters : a Publication of the IEEE Laser and Electro-Optics Society
|February 2, 2018
PubMed
Summary
This summary is machine-generated.

We developed a simple, low-maintenance quantitative phase microscope. This novel microscope uses a self-referencing technique for high-accuracy imaging of biological samples, offering variable magnification and low noise.

Keywords:
Bioinstrumentationinterferometryoptical imagingphase retrieval

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Area of Science:

  • Optical microscopy
  • Biomedical imaging
  • Quantitative phase imaging

Background:

  • Quantitative phase microscopy (QPM) provides label-free contrast for transparent specimens.
  • Traditional QPM setups can be complex and sensitive to environmental disturbances.
  • There is a need for robust and user-friendly QPM systems for biological applications.

Purpose of the Study:

  • To present a novel near-common-path self-reference quantitative phase microscope.
  • To demonstrate its capability for high-accuracy, variable-magnification imaging of biological samples.
  • To highlight its simple, maintenance-free optical design for accessibility.

Main Methods:

  • Utilizing off-axis interference between a sample wave and its 180-degree rotated counterpart.
  • Employing a pair of dove prisms with differing orientations for beam transformation.
  • Implementing a near-common-path optical design for enhanced stability.

Main Results:

  • Successful formation of quantitative phase images through self-interference.
  • Demonstrated variable magnification suitable for diverse sample sizes.
  • Achieved low-noise phase measurements for high-accuracy imaging.
  • The optical design requires no maintenance, simplifying operation.

Conclusions:

  • The developed quantitative phase microscope is robust, user-friendly, and maintenance-free.
  • Its capabilities make it ideal for high-accuracy imaging of biological samples.
  • This technique offers an accessible solution for quantitative phase imaging in various research settings.