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Updated: Oct 14, 2025

Multicolor Fluorescence Detection for Droplet Microfluidics Using Optical Fibers

Multicolor Fluorescence Detection for Droplet Microfluidics Using Optical Fibers

Published on: May 5, 2016

Free-Space Excitation of Optofluidic Devices for Pattern-Based Single Particle Detection.

Md Nafiz Amin¹, Vahid Ganjalizadeh¹, Matt Hamblin²

¹School of Engineering, University of California Santa Cruz, Santa Cruz, CA 95064 USA.

IEEE Photonics Technology Letters : a Publication of the IEEE Laser and Electro-Optics Society

|November 8, 2021

Summary

This summary is machine-generated.

This study introduces novel top-down illumination for optofluidic sensors, enhancing single-particle detection in liquid-core antiresonant reflecting optical waveguides (ARROW). These methods offer improved signal-to-noise ratios and relaxed alignment for chip-based sensing.

Keywords:

Antiresonant reflecting optical waveguides (ARROW)biophotonics fluorescence sensing optofluidics single particle detection

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Last Updated: Oct 14, 2025

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Published on: December 10, 2011

Microfluidic Platform with Multiplexed Electronic Detection for Spatial Tracking of Particles

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Area of Science:

Optics and Photonics
Biomedical Engineering
Materials Science

Background:

Optofluidic sensors utilize planar waveguide excitation for single molecule detection.
Existing methods often require complex in-plane excitation schemes.

Purpose of the Study:

To demonstrate a new top-down illumination approach for single-particle fluorescence sensing.
To evaluate two distinct multi-spot excitation techniques for liquid-core antiresonant reflecting optical waveguide (ARROW) devices.

Main Methods:

Free-space, top-down illumination of liquid-core ARROW devices.
Excitation via a slit pattern in an opaque film.
Direct imaging of a Y-splitter waveguide spot pattern onto the sensor.

Main Results:

Comparable performance to in-plane excitation for single bead detection using the slit pattern method.
A 2.7x improvement in signal-to-noise ratio with the direct imaging technique.
Demonstrated feasibility of top-down illumination for optofluidic particle sensing.

Conclusions:

Top-down illumination offers a viable and potentially superior alternative for optofluidic sensing.
These methods simplify chip-based optical particle sensing with relaxed alignment.
The direct imaging technique shows significant potential for enhanced signal detection.