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Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

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Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
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Confocal Fluorescence Microscopy01:16

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Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
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Total Internal Reflection Fluorescence Microscopy01:05

Total Internal Reflection Fluorescence Microscopy

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Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.
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Imaging Biological Samples with Optical Microscopy01:18

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Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
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Chromatographic Resolution01:15

Chromatographic Resolution

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In chromatography, a solute moves through a chromatographic column and tends to spread, forming a Gaussian-shaped band. The longer the solute spends in the column, the broader the band becomes. The broadening can lead to overlaps within the column, affecting separation effectiveness.
The effectiveness of separation can be evaluated by determining the level of separation between two neighboring peaks in a chromatogram, which represents the individual components of a sample.
In chromatography,...
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Cancers Originate from Somatic Mutations in a Single Cell02:21

Cancers Originate from Somatic Mutations in a Single Cell

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Cancer arises from mutations in the critical genes that allow healthy cells to escape cell cycle regulation and acquire the ability to proliferate indefinitely. Though originating from a single mutation event in one of the originator cells, cancer progresses when the mutant cell lines continue to gain more and more mutations, and finally, become malignant. For example, chronic myelogenous leukemia (CML) develops initially as a non-lethal increase in white blood cells, which progressively...
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Related Experiment Video

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Sample Preparation for Single Virion Atomic Force Microscopy and Super-resolution Fluorescence Imaging
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Super-Resolution Fluorescence Microscopy for Single Cell Imaging.

Han Feng1, Xiaobo Wang2,3, Zhiwei Xu4

  • 1Department of Pharmacy, Henan Provincial People's Hospital, and People's Hospital of Zhengzhou University, Zhengzhou, China.

Advances in Experimental Medicine and Biology
|June 27, 2018
PubMed
Summary
This summary is machine-generated.

Super-resolution microscopy, including STED, SIM, PALM, and STORM, offers advanced nanoscale imaging for biological research. These techniques provide deeper insights into single-cell dynamics and structures.

Keywords:
PALMSIMSTED microscopySTORMSingle cell imaging

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Conventional BODIPY Conjugates for Live-Cell Super-Resolution Microscopy and Single-Molecule Tracking
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Area of Science:

  • Cellular Biology
  • Microscopy Techniques
  • Biophysics

Background:

  • Super-resolution fluorescence microscopy has rapidly advanced over the last 20 years.
  • Techniques like STED, SIM, PALM, and STORM overcome traditional light microscopy resolution limits.

Purpose of the Study:

  • To review the principles and recent applications of super-resolution microscopy in single-cell imaging.
  • To discuss advancements in 3D, multicolor, live-cell, and multimodal super-resolution imaging.

Main Methods:

  • Stimulated Emission Depletion (STED) microscopy
  • Structured Illumination Microscopy (SIM)
  • Photoactivation Localization Microscopy (PALM)
  • Stochastic Optical Reconstruction Microscopy (STORM)

Main Results:

  • Super-resolution techniques enable imaging of nanoscale structures and fast cellular dynamics.
  • Extensions to 3D, multicolor, and live-cell imaging significantly enhance precision.
  • Integration with other fields expands imaging capabilities.

Conclusions:

  • Super-resolution microscopy provides comprehensive insights into single-cell details.
  • Continued development promises further breakthroughs in biological research.