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Therapeutic Antibody Discovery in Infectious Diseases Using Single-Cell Analysis
Alexandria Voigt1, Touyana Semenova1, Janet Yamamoto1
1Department of Infectious Diseases and Pathology, College of Veterinary Medicine, University of Florida, Gainesville, FL, USA.
Advances in Experimental Medicine and Biology
|June 27, 2018
Summary
Monoclonal antibody discovery has advanced with hybridoma and phage display. Single-cell analysis now identifies therapeutic antibodies for diseases like HIV and Zika virus.
Area of Science:
- Immunology and Biotechnology
- Single-cell analysis
- Therapeutic antibody development
Background:
- Hybridoma technology (1975) revolutionized monoclonal antibody production.
- Advances in B cell immortalization and phage display yielded numerous diagnostic and therapeutic antibodies.
- Omics technologies revealed cellular heterogeneity, emphasizing single-cell functional individuality.
Purpose of the Study:
- To review traditional antibody discovery methods.
- To explore recent single-cell antibody discovery platforms.
- To discuss single-cell analysis applications for therapeutic antibodies against HIV and Zika virus.
Main Methods:
- Review of established antibody discovery techniques.
- Analysis of emerging single-cell technologies for antibody isolation.
- Case studies on therapeutic antibody discovery for viral infections.
Main Results:
- Single-cell analysis enables precise identification of functional B cells.
- New platforms accelerate the discovery of therapeutic monoclonal antibodies.
- Successful applications demonstrated for HIV and Zika virus antibody development.
Conclusions:
- Single-cell analysis is pivotal for discovering novel therapeutic antibodies.
- Technological integration enhances efficiency in antibody discovery pipelines.
- This approach holds significant promise for combating infectious diseases.
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