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pH-Operated Triplex DNA Device on MoS2 Nanosheets.
1Shanghai Key Laboratory of Green Chemistry and Chemical Processes, School of Chemistry and Molecular Engineering , East China Normal University , 500 Dongchuan Road , Shanghai 200241 , P. R. China.
Langmuir : the ACS Journal of Surfaces and Colloids
|March 19, 2019
Summary
A novel DNA device using triplex DNA and molybdenum disulfide (MoS2) nanosheets functions as a pH sensor. This biosensor platform shows reversible fluorescence signals in response to pH changes.
Area of Science:
- Biotechnology
- Nanotechnology
- Molecular Biology
Background:
- DNA nanostructures offer versatile platforms for biosensing applications.
- Molybdenum disulfide (MoS2) nanosheets possess unique electronic and optical properties suitable for signal transduction.
- Developing sensitive and reversible pH-sensing platforms is crucial for various biological and environmental monitoring applications.
Purpose of the Study:
- To develop and characterize a novel pH-sensing platform utilizing a triplex DNA device integrated with molybdenum disulfide (MoS2) nanosheets.
- To investigate the pH-dependent conformational changes of the DNA device and their correlation with fluorescence signals.
- To assess the reversibility and performance of the MoS2-enhanced DNA biosensor.
Main Methods:
- Fabrication of a triplex-based DNA device designed to transition between duplex and triplex states at different pH values.
- Integration of the DNA device with molybdenum disulfide (MoS2) nanosheets.
- Utilizing a pH-insensitive fluorescent dye to generate a signal modulated by the DNA-MoS2 interaction.
- Characterization of the DNA device's operation, pH response, and reversibility on the MoS2 nanosheet platform.
Main Results:
- The DNA device exhibited a conformational transition from a duplex to a triplex state between pH 8 and pH 5.
- The interaction between the DNA device and MoS2 nanosheets generated distinct fluorescence signals corresponding to the different pH states.
- The pH-sensing platform demonstrated reversibility, indicating its potential for continuous monitoring.
- Successful characterization of the DNA device operation and pH response on the MoS2 nanosheet platform.
Conclusions:
- A functional pH-sensing platform was successfully developed by coupling a triplex DNA device with MoS2 nanosheets.
- The MoS2-enhanced DNA device provides a sensitive and reversible method for pH detection via fluorescence signaling.
- This strategy opens avenues for creating a new generation of biosensors employing triplex DNA elements modulated by inorganic nanomaterials.
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