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Reliability and Analyst Performance Limits for the Field and Single Strip Direct Microscopic Somatic Cell Count
James E Leslie1, Jerald E Barnett1, Helen K Bachelor1
1Division of Microbiology, Food and Drug Administration, 1090 Tusculum Avenue, Cincinnati, Ohio 45226.
Journal of Food Protection
|March 29, 2019
Summary
The single strip direct microscopic somatic cell count (DMSCC) procedure is more reliable and less variable than the field count method. This finding is crucial for accurate somatic cell count analysis in dairy herds.
Area of Science:
- Dairy Science
- Animal Health
- Microbiology
Background:
- Somatic cell count (SCC) is a key indicator of udder health in dairy animals.
- Accurate SCC measurement is vital for herd management and milk quality.
- Direct microscopic somatic cell count (DMSCC) is a common method for determining SCC.
Purpose of the Study:
- To compare the reliability and variability of two DMSCC methods: field count and single strip.
- To evaluate the performance of each method across different SCC ranges.
Main Methods:
- Comparison of means and total components of variance between field and single strip DMSCC procedures.
- Statistical analysis of variance components and coefficients of variation.
Main Results:
- The field count procedure averaged 12-28% higher than the single strip procedure within the 300,000 to 1,200,000 DMSCC/ml range.
- The field procedure showed a higher sum of variance components (0.01058) and coefficient of variation (24%) compared to the single strip procedure (0.00834 and 21%).
Conclusions:
- The single strip DMSCC procedure provides more reliable and less variable results than the field count procedure.
- This suggests the single strip method is preferable for accurate SCC determination in dairy diagnostics.
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