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[PECULIARITIES OF IMMUNE PROFILE IN BLOOD OF RATS IN THE DYNAMICS OF IMMOBILIZATION STRESS ON THE BACKGROUND OF

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Immobilization stress impacts immune cells, decreasing T-lymphocytes and increasing B-lymphocytes. Hypothyroidism exacerbates this immune decline during stress, highlighting the critical role of thyroid hormones in immune regulation.

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Area of Science:

  • Immunology
  • Endocrinology
  • Stress Physiology

Background:

  • Immobilization stress is a known physiological disruptor.
  • Hypothyroidism, a deficiency in thyroid hormones, can affect various bodily systems.
  • The interplay between stress and immune function, particularly under endocrine disruption, requires further elucidation.

Purpose of the Study:

  • To investigate alterations in cellular and humoral immunity during immobilization stress.
  • To examine how hypothyroidism influences the immune response to immobilization stress.
  • To assess changes in lymphocyte populations and the immunoregulatory index under these conditions.

Main Methods:

  • Utilized 84 male Wistar rats for the study.
  • Induced immobilization stress to model the stress response.
  • Analyzed cellular and humoral immunity indices using flow cytometry (Beckman Coulter Epics-XL).

Main Results:

  • Immobilization stress led to a significant decrease in cellular immunity indices across anxiety, resistance, and depletion stages.
  • T-lymphocyte levels decreased, while B-lymphocyte populations increased by 26.5% during the anxiety stage.
  • Hypothyroidism significantly reduced both cellular and humoral immunity at all stress stages, lowering the immunoregulatory index (p<0.05).

Conclusions:

  • Immobilization stress profoundly impacts cellular immunity, with distinct changes in lymphocyte populations.
  • Hypothyroidism exacerbates the immunosuppressive effects of immobilization stress.
  • Thyroid hormone status is crucial for maintaining immune homeostasis during stress.