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Fabrication of Three-dimensional Paper-based Microfluidic Devices for Immunoassays
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Sampling and multiplexing in lab-on-paper bioelectroanalytical devices for glucose determination.
O Amor-Gutiérrez1, E Costa-Rama1, M T Fernández-Abedul1
1Departamento de Química Física y Analítica, Facultad de Química, Universidad de Oviedo, 33006, Oviedo, Spain.
Biosensors & Bioelectronics
|April 19, 2019
Summary
We developed a low-cost, multiplexed paper-based electrochemical device for rapid glucose determination. This simple biosensor integrates sampling, advancing lab-on-a-chip technology for accurate analysis.
Area of Science:
- Electrochemistry
- Biosensors
- Analytical Chemistry
Background:
- Paper-based electrochemical devices offer low-cost, portable analytical platforms.
- Integrating sampling steps enhances usability for point-of-care applications.
- Multiplexed measurements increase analytical throughput.
Purpose of the Study:
- To develop a simple, low-cost, multiplexed paper-based electrochemical device for glucose determination.
- To integrate a sampling step using a glass-fiber strip for enhanced usability.
- To validate the device's performance for glucose analysis in real samples.
Main Methods:
- Fabrication of paper-based electrodes using carbon ink and multifunctional connectors.
- Immobilization of enzymes (glucose oxidase, horseradish peroxidase) and ferrocyanide mediator.
- Development of a microfluidic sampling approach using glass-fiber strips.
- Electrochemical measurements via chronoamperometry for glucose quantification.
Main Results:
- Achieved a linear range of 0.5–15 mM for glucose determination with high precision.
- Demonstrated excellent reproducibility with a relative standard deviation (RSD) of approximately 1% for calibration slopes.
- Obtained accurate glucose measurements in real samples like orange fruit and cola beverages.
Conclusions:
- The developed paper-based electrochemical device is a simple, low-cost, and effective platform for multiplexed glucose sensing.
- The integrated sampling step eliminates the need for micropipettes, improving ease of use.
- This approach represents a significant step towards practical lab-on-a-chip devices for various analytical applications.
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