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A Facile, Versatile, and Highly Efficient Strategy for Peroxynitrite Bioimaging Enabled by Formamide Deformylation.
Xilei Xie1, Guangzhao Liu1, Xingxing Su1
1College of Chemistry, Chemical Engineering and Materials Science, Key Laboratory of Molecular and Nano Probes, Ministry of Education, Collaborative Innovation Center of Functionalized Probes for Chemical Imaging in Universities of Shandong , Shandong Normal University , Jinan 250014 , People's Republic of China.
Analytical Chemistry
|April 20, 2019
Summary
Researchers developed a new, versatile strategy for peroxynitrite (ONOO-) bioimaging using amine formylation/deformylation chemistry. This method enables rapid, sensitive, and specific visualization of ONOO- in live cells and animal models.
Area of Science:
- Chemical Biology
- Biomedical Imaging
- Reactive Oxygen Species
Background:
- Peroxynitrite (ONOO-) is implicated in various pathophysiological processes.
- Current ONOO- bioimaging faces challenges including lack of specific triggers, complex probe synthesis, and limited versatile strategies.
Purpose of the Study:
- To develop a novel, facile, and versatile chemical strategy for peroxynitrite (ONOO-) bioimaging.
- To design and synthesize novel fluorescent probes for specific and sensitive ONOO- detection.
Main Methods:
- Development of a bioimaging strategy based on amine formylation/deformylation chemistry.
- Design, synthesis, and evaluation of four novel formamide-functionalized fluorescent probes.
- Demonstration of probe utility in visualizing ONOO- fluctuations in live cells and mouse organs.
Main Results:
- A one-step synthesis yielding four novel fluorescent probes with high efficiency.
- Probes exhibited specific, sensitive, and rapid responses to peroxynitrite (ONOO-).
- Successful visualization of ONOO- in live cells and in organs of paraquat-poisoned mice.
Conclusions:
- The amine formylation/deformylation strategy offers a facile, versatile, and efficient approach for peroxynitrite (ONOO-) visualization.
- This methodology significantly advances ONOO- biochemistry and pathophysiology research.
- The developed probes are suitable for real-time monitoring of ONOO- in biological systems.
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