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Published on: August 25, 2022
Secretome of Cultured Human Endothelial Cells in Simulated Microgravity
D N Kashirina1, A S Kononikhin2, I M Larina2
1State Research Center Institute of Biomedical Problems, Russian Academy of Sciences, Moscow, Russia. daryakudryavtseva@mail.ru.
Abstract:
The secretome of human umbilical vein endothelial cells (HUVEC) cultured under static conditions and in modeled microgravity for 24 h was studied by chromatography-mass spectrometry. In the secretome of cells exposed to microgravity, we identified a group of microtubule proteins including many structural elements of microtubules and regulatory proteins interacting with Rho-GTPases. Hence, reorganization of actin cytoskeleton and microtubules induced by microgravity is under complex regulation mediated by Rho proteins.
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