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Identification of Functional Protein Regions Through Chimeric Protein Construction
Published on: January 8, 2019
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Fusion proteins with chromogenic and keratin binding modules
Ana Tinoco1, Egipto Antunes1, Madalena Martins1
1CEB - Centre of Biological Engineering, University of Minho, Campus de Gualtar, 4710-057, Braga, Portugal.
Scientific Reports
|October 3, 2019
Summary
A novel fusion protein, keratin-based peptide-ultramarine (KP-UM), acts as an eco-friendly hair dye, coloring and strengthening hair while demonstrating safety. This innovative cosmetic ingredient offers a sustainable alternative to traditional hair colorants.
Area of Science:
- Biotechnology
- Cosmetic Science
- Materials Science
Background:
- Traditional hair dyes pose environmental and health concerns.
- Developing safe and sustainable hair coloration agents is a priority.
- Keratin-based peptides offer hair affinity and potential for functionalization.
Purpose of the Study:
- To develop and evaluate a novel fusion protein for hair coloration.
- To investigate the chromogenic properties and hair binding of the fusion protein.
- To assess the safety and efficacy of the fusion protein as a hair coloring agent.
Main Methods:
- Construction of a fusion protein: chromogenic ultramarine protein (UM) linked to a keratin-based peptide (KP).
- Application of the KP-UM protein to overbleached hair to assess color deposition and penetration.
- Molecular dynamics simulations to elucidate protein-hair interactions.
- Mechanical property testing of treated hair and in vitro safety assessment using human keratinocytes.
Main Results:
- The KP-UM fusion protein effectively colored overbleached hair, with color intensity dependent on formulation polarity.
- The protein demonstrated strong binding to the hair cuticle and penetration into the hair fiber, mediated by the KP sequence.
- Formulations restored mechanical properties of overbleached hair.
- KP-UM protein exhibited photoswitching behavior and was found safe on human keratinocytes.
Conclusions:
- The KP-UM fusion protein is a viable, safe, and eco-friendly candidate for hair coloration.
- This protein offers a sustainable alternative to conventional hair dyes, reducing environmental impact.
- The dual chromogenic and photoswitching properties present unique opportunities for cosmetic applications.
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