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Updated: Jan 5, 2026

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Quantifying Yeast Chronological Life Span by Outgrowth of Aged Cells
Published on: May 6, 2009
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Comparison of microplate- and bottle-based methods to age yeast for chronological life span assays
Mandy Mun Yee Kwong1, Jee Whu Lee1, Mohammed Razip Samian2
1Institute for Research in Molecular Medicine (INFORMM), Universiti Sains Malaysia, Malaysia; USM-RIKEN International Centre for Ageing Science (URICAS), Universiti Sains Malaysia, Malaysia.
Journal of Microbiological Methods
|October 20, 2019
Abstract:
This study compared the chronological life span and survival of Saccharomyces cerevisiae aged in a microplate or bottle, under different aeration and calorie restriction conditions. Our data shows that limited aeration in the microplate-aged culture contributed to slower outgrowth but extended yeast CLS compared to the bottle-aged culture.

